Re: Oil in a 10x objective

Posted by Goodhouse, Joseph G. on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Oil-in-a-10x-objective-tp2545827p2546171.html

Storing lenses.  Recently I was informed by one of my microscope technical people that the proper way to store optics is the same way the are mounted on your microscope.  That is lens down for an upright microscope and lens up for an inverted microscope.  This is to prevent immersion fluids from getting into the lens ( around the fluid barriers)  by gravitational and capillary action.

 

Joe Goodhouse
Confocal Core Lab Manager
Dept. of Molecular Biology
Princeton University
609-258-5432

Visit us at http://www.molbio1.princeton.edu/facility/confocal/  

 

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From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Craig Brideau
Sent: Friday, March 27, 2009 3:00 PM
To: [hidden email]
Subject: Re: Oil in a 10x objective

All our objective bottles actually store them this way.  The lens screws into the lid of the bottle and points down.

Craig

On Fri, Mar 27, 2009 at 12:22 PM, Gary Laevsky <[hidden email]> wrote:

A great wise one once told me to always store objectives in their case
with back aperture up, so just in case oil didn't drip into the body if
stored back aperture down.

Best,



Gary Laevsky, Ph.D.

Imaging Application Specialist



Andor Technology

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[hidden email]

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-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]]
On Behalf Of Steven Ruzin

Sent: Friday, March 27, 2009 2:14 PM
To: [hidden email]
Subject: Oil in a 10x objective

Can anyone come up with a plausible explanation as to how oil got
into the 10x objective on our LSM510 with an upright stand? Yesterday
someone complained about poor imaging through the objective, and I
discovered that there was a bubble of oil trapped between internal
elements. I'm at a loss as to how someone got oil inside this low
mag, non-immersion lens. Any ideas?

Steve...
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________________________________________________________________________
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Steven E. Ruzin, Ph.D.
Director, Biological Imaging Facility
Curator, Golub Microscope Collection
381 Koshland Hall               College of Natural Resources
University of California        Berkeley CA 94720-3102
510-642-6602                    510-642-4995 (fax)
http://golubcollection.berkeley.edu
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