Re: Oil in a 10x objective
Posted by
Dan Focht on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Oil-in-a-10x-objective-tp2545827p2547011.html
Steve
This is not an answer but it might put a smile on your face, if you have a sick sense of humor.
When I serviced Zeiss microscopes, in a former life, I had a client at a major University that asked to have their "immersion scope" serviced.
When I got there I found that a foreign speaking student had poured a whole bottle of immersion oil into the support arm of the Zeiss Photo III microscope.
If his English comprehension would have been a bit better, it would not have happened.
It was a mess. The camera mechanism, connectors, optics, and PMT was soaked.
I hope the repair cost of your 10X is not anywhere near the cost they had to endure!
Dan
On Mar 27, 2009, at 2:13 PM, Steven Ruzin wrote:
Can anyone come up with a plausible explanation as to how oil got into the 10x objective on our LSM510 with an upright stand? Yesterday someone complained about poor imaging through the objective, and I discovered that there was a bubble of oil trapped between internal elements. I'm at a loss as to how someone got oil inside this low mag, non-immersion lens. Any ideas?
Steve...
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____________________________________________________________________________
Steven E. Ruzin, Ph.D.
Director, Biological Imaging Facility
Curator, Golub Microscope Collection
381 Koshland Hall College of Natural Resources
University of California Berkeley CA 94720-3102
510-642-6602 510-642-4995 (fax)
Dan Focht
Bioptechs
724-282-7145
www.bioptechs.com