Cleaning lens.

Posted by Ian Montgomery on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cleaning-lens-tp2556737.html

            In one of our teaching labs many years ago a student complained they were having a problem with the x100 OI objective and sure enough the image was lousy. I cleaned the objective and slide then re-applied a spot of oil and still the image was lousy. I then asked the student how exactly they had set up the microscope. Shock horror, my world collapsed. They had unscrewed the objective, filled it with oil, screwed it back on then put a drop on the slide. After weeks of trying to clean the objective it went into the trash as beyond economic repair.

            Cleaning objectives, I use the fluid recommended by the local Zeiss engineer, 90% methylated spirit and 10% isopropanol.

Ian.   

 

Dr. Ian Montgomery,

Histotechnology,

I.B.L.S. Support Unit,

Thomson Building,

University of Glasgow,

Glasgow,

G12 8QQ.