Membrane specific dye to be used in IHC

Posted by Kim Anker Kristiansen on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cleaning-lens-tp2556737p2557125.html

Dear Listers,

 

I am looking for a membrane specific dye to use together with antibodies in IHC staining. We would like to set up a system where we look at receptors associated with the cell membrane, hence the membrane dye.

 

We are doing cryo-sectioning, acetone fixation and TBS buffered immunostaining.

 

So my question is: What would be a good choice of dye to apply to the already immunostained tissue?

 

Cheers Kim

 

Kim A. Kristiansen
Post Doc.
Glostrup Research Center
Department of Clinical Research
Glostrup Hospital
Ndr Ringvej 69
DK-2600 Glostrup
Denmark
Tel: [phone]
E-mail: [hidden email]