Posted by
Fredrik Wermeling on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cleaning-lens-tp2556737p2557595.html
I know that people use cholera-toxin B-subunit conjugates to label lipid rafts. I think you can find it conjugated to several alexa dyes. /fredrik
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Fredrik Wermeling, PhD-student
Karolinska Institutet
Department of Medicine
Unit of Clinical Allergy Research
L2:04 Karolinska Hospital
SE-171 76 Stockholm
Sweden
phone: +46-8-51776696
mail:
[hidden email] fax: +46-8-335724
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----- Original Message -----
From: Kim Anker Kristiansen <
[hidden email]>
Date: Monday, March 30, 2009 3:07 pm
Subject: Membrane specific dye to be used in IHC
To:
[hidden email] > Dear Listers,
>
>
>
> I am looking for a membrane specific dye to use together with
> antibodiesin IHC staining. We would like to set up a system where
> we look at
> receptors associated with the cell membrane, hence the membrane dye.
>
>
>
> We are doing cryo-sectioning, acetone fixation and TBS buffered
> immunostaining.
>
>
>
> So my question is: What would be a good choice of dye to apply to the
> already immunostained tissue?
>
>
>
> Cheers Kim
>
>
>
> Kim A. Kristiansen
> Post Doc.
> Glostrup Research Center
> Department of Clinical Research
> Glostrup Hospital
> Ndr Ringvej 69
> DK-2600 Glostrup
> Denmark
> Tel: +45 25219914
> E-mail:
[hidden email] >
>
>
>