We use a WGA(wheat germ agglutinin) - ALEXA conjugate
(Invitrogen) in muscle cross-sections.
We apply this after our primary and secondary and get beautiful
results.
___________________________________________
Christopher W. Ward Ph.D.
Associate Professor
University of Maryland Baltimore: School of Nursing
655 W. Lombard St.
Rm 752
Baltimore MD 21201
Ph: 410.706.3618
Fax: 410.706.0399
Skype: chrisward_umbaltimore
From: Confocal Microscopy
List [mailto:[hidden email]] On Behalf Of Fredrik
Wermeling
Sent: Monday, March 30, 2009 10:44 AM
To: [hidden email]
Subject: Re: Membrane specific dye to be used in IHC
I know that people use cholera-toxin B-subunit conjugates to
label lipid rafts. I think you can find it conjugated to
several alexa dyes. /fredrik
--------------------------------------
Fredrik Wermeling, PhD-student
Karolinska Institutet
Department of Medicine
Unit of Clinical Allergy Research
L2:04 Karolinska Hospital
SE-171 76 Stockholm
Sweden
phone: +46-8-51776696
mail: [hidden email]
fax: +46-8-335724
--------------------------------------
----- Original Message -----
From: Kim Anker Kristiansen <[hidden email]>
Date: Monday, March 30, 2009 3:07 pm
Subject: Membrane specific dye to be used in IHC
To: [hidden email]
> Dear Listers,
>
>
>
> I am looking for a membrane specific dye to use together with
> antibodiesin IHC staining. We would like to set up a system where
> we look at
> receptors associated with the cell membrane, hence the membrane dye.
>
>
>
> We are doing cryo-sectioning, acetone fixation and TBS buffered
> immunostaining.
>
>
>
> So my question is: What would be a good choice of dye to apply to the
> already immunostained tissue?
>
>
>
> Cheers Kim
>
>
>
> Kim A. Kristiansen
> Post Doc.
> Glostrup Research Center
> Department of Clinical Research
> Glostrup Hospital
> Ndr Ringvej 69
> DK-2600 Glostrup
> Denmark
> Tel: +45 25219914
> E-mail: [hidden email]
>
>
>
>
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