Re: Membrane specific dye to be used in IHC

Posted by Jean-Pierre CLAMME-2 on Mar 30, 2009; 3:59pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Cleaning-lens-tp2556737p2558020.html


Is WGA specific to plasma membrane ?



Confocal Microscopy List <[hidden email]> wrote on 03/30/2009 07:47:09 AM:

> We use a WGA(wheat germ agglutinin) - ALEXA  conjugate (Invitrogen)
> in muscle cross-sections.
>
> We apply this after our primary and secondary and get beautiful results.
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> ___________________________________________
>
> Christopher W. Ward Ph.D.
>
> Associate Professor
>
> University of Maryland Baltimore:  School of Nursing
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> 655  W. Lombard St.
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> Rm 752
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> Baltimore MD 21201
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> Ph:   410.706.3618
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> Fax:  [phone]
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> Skype:  chrisward_umbaltimore
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> From:Confocal Microscopy List [mailto:[hidden email]]
> On Behalf Of Fredrik Wermeling
> Sent: Monday, March 30, 2009 10:44 AM
> To: [hidden email]
> Subject: Re: Membrane specific dye to be used in IHC
>
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> I know that people use cholera-toxin B-subunit conjugates to label
> lipid rafts. I think you can find it conjugated to several alexa
> dyes. /fredrik
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> --------------------------------------
> Fredrik Wermeling, PhD-student
> Karolinska Institutet
> Department of Medicine
> Unit of Clinical Allergy Research
> L2:04 Karolinska Hospital
> SE-171 76 Stockholm
> Sweden
>
> phone: [phone]
> mail: [hidden email]
> fax: [phone]
> --------------------------------------
>
> ----- Original Message -----
> From: Kim Anker Kristiansen <[hidden email]>
> Date: Monday, March 30, 2009 3:07 pm
> Subject: Membrane specific dye to be used in IHC
> To: [hidden email]
>
> > Dear Listers,
> >
> >
> >
> > I am looking for a membrane specific dye to use together with
> > antibodiesin IHC staining. We would like to set up a system where
> > we look at
> > receptors associated with the cell membrane, hence the membrane dye.
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> > We are doing cryo-sectioning, acetone fixation and TBS buffered
> > immunostaining.
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> > So my question is: What would be a good choice of dye to apply to the
> > already immunostained tissue?
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> > Cheers Kim
> >
> >
> >
> > Kim A. Kristiansen
> > Post Doc.
> > Glostrup Research Center
> > Department of Clinical Research
> > Glostrup Hospital
> > Ndr Ringvej 69
> > DK-2600 Glostrup
> > Denmark
> > Tel: [phone]
> > E-mail: [hidden email]
> >
> >
> >
> >