http://confocal-microscopy-list.275.s1.nabble.com/microbead-calibration-to-set-psf-tp2560403p2560487.html
> If you want to roll your own, use tetraspec beads 100nm and a 1000:1
> lower concentration of 200nm beads. Dry them onto a coverslip and mount.
>
> Molecular probes, aka Invitrogen, has a prepared bead slide that works
> as well.
>
> Why don't you ask Autoquant? They should be the experts in collecting
> psf's with their software.
>
> Tom
>
> Tom Donnelly
> Applied Precision, Inc.
> 1040 12th Ave. N.W. Issaquah, WA 98027-8929
> (425)829-9414 (425)557-1055 fax
>
[hidden email]
>
http://www.api.com/lifescience/DeltaVision.html>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:
[hidden email]]
> On Behalf Of Matiar Jafari
> Sent: Monday, March 30, 2009 4:59 PM
> To:
[hidden email]
> Subject: microbead calibration to set psf?
>
> hey again,
>
> I'm trying to find out how and what the best way to setup my own psf is
> in AutoQuant. I know i need microbeads and im wondering what brand and
> what product i need and then how i need to shoot it and load it.
> Also what diameter does it need to be. A cat# would be great too also i
> don't know if it matters on what im shooting but just to put it out
> there im shooting gfp slices. Sorry if this is vague. Any help would be
> greatly appreciated. Thanks in advance
>
> Thank You
> --
> Matiar Jafari
>
>
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