Posted by
M. van de corput on
URL: http://confocal-microscopy-list.275.s1.nabble.com/microbead-calibration-to-set-psf-tp2560403p2561606.html
I have good experience with fluorescent beads from Duke scientific. The
100nm beads are very bright and are perfect for determining the PSF. I use
the red, green and blue 100nm beads to determine the PSF in three channels
and to deconvolved my 3 colour FISH images.
I have trouble finding the 100nm and 200nm TetraSpeck beads on my slide
beacuse they are rather weak in intensity. So that's why I switched to the
single coloured 100nm beads from DS. The 500nm TetraSpeck beads are very
good and I use them to determine the chromatic shift in 4 channels. Once
made, bead slides can be used for a long period of time.
Mariette
____________________________________________
Dr. M.P.C. Kemner-van de Corput,
____________________________________________
MGC - Dept. of Cell Biology & Genetics
Erasmus Medical Center
Dr. Molewaterplein 50, 3015 GE Rotterdam
POB 2040, 3000 CA Rotterdam, The Netherlands
Office: H-Ee751; tel: +31 10 704.3949
Lab: H-Ee710; tel lab: +31 10 704.3315
tel secr: +31 10 704.3169
____________________________________________
http://www2.eur.nl/fgg/ch1/cellbiology/http://www.thesis.kemner.biz/____________________________________________
Op Di, 31 maart, 2009 1:59 am, schreef Matiar Jafari:
> hey again,
>
> I'm trying to find out how and what the best way to setup my own psf
> is in AutoQuant. I know i need microbeads and im wondering what brand
> and what product i need and then how i need to shoot it and load it.
> Also what diameter does it need to be. A cat# would be great too also
> i don't know if it matters on what im shooting but just to put it out
> there im shooting gfp slices. Sorry if this is vague. Any help would
> be greatly appreciated. Thanks in advance
>
> Thank You
> --
> Matiar Jafari
>