Re: microbead calibration to set psf?

Posted by Jean-Pierre CLAMME-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/microbead-calibration-to-set-psf-tp2560403p2563846.html


Hi,

I just used PS-Speck Beads from Molecular Probes and  they worked just fine for me.  They are brighter than the Tetra Speck but single color.

JP
 


Confocal Microscopy List <[hidden email]> wrote on 03/30/2009 04:59:05 PM:

> hey again,

> I'm trying to find out how and what the best way to setup my own psf
> is in AutoQuant.  I know i need microbeads and im wondering what brand
> and what product i need and then how i need to shoot it and load it.
> Also what diameter does it need to be.  A cat# would be great too also
> i don't know if it matters on what im shooting but just to put it out
> there im shooting gfp slices.  Sorry if this is vague. Any help would
> be greatly appreciated.  Thanks in advance

> Thank You
> --
> Matiar Jafari