Ultra clean cover glass and HELA transfections

Posted by vb-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/microbead-calibration-to-set-psf-tp2560403p2569363.html

Dear All,

 

I am looking for a supplier of Ultra Clean Cover glass (#1.5 and/or #2) (Ultra Clean stands for chemically clean, i.e. treated with a piranha solution (Sulfuric acid + hydrogen peroxide), etc.

 

Second, would you be willing to share your experience on the high efficiency transfection of HELA cells (>40%) at low confluency (30-40%), which HELA reagent would reveal the lowest cytotoxicity.

 

Thank you,

 

Vitaly

NCI-Frederick,

301-846-6575