Re: Ultra clean cover glass and HELA transfections

Posted by Florian Eich on
URL: http://confocal-microscopy-list.275.s1.nabble.com/microbead-calibration-to-set-psf-tp2560403p2573627.html

Hello Vitaly,
during my work in the lab I found Metafectene Pro a very good transfectant
for HELA cells, regardless of the confluency.
But it mainly depended also on very clean plasmid-preps which sometimes was
more difficult than the transfection.
Also worth a try if you are unsatisfied with "normal" transfection would be to
do Elektroporation with Amaxa, this also worked nicely for my experiments, and
since you have the cells in suspension when you do the transfection you can
select the number you seed and then get the desired confluency.
To try Amaxa, just contact them. In Germany they sometimes supply you with
a machine to test their system.

No commercial interest for both companies mentioned.

Good Luck

Florian