FRET results

Posted by ramesh kandpal on
URL: http://confocal-microscopy-list.275.s1.nabble.com/FRET-results-tp2580297.html

hi,

 

can anyone give some idea that which is the most appropriate laser wavelength for performing acceptor photobleaching method of CFP/YFP FRET. If 488nm laser is used for YFP excitation is there any possibility of loosing FRET data. What difference does it create  in FRET results if we use 514nm laser line.

 

thanks & best wishes,

 

RK