Re: Microinjection pipettes for microbeads

Posted by Martin Wessendorf-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Microinjection-pipettes-for-microbeads-tp2580788p2580930.html

Dear Kris--

Rather than try to buy what you need, you might just go over to the
Physiology Department in the medical school and ask who the
electrophysiologists are.  An electrophysiologist will have devices that
can pull pipettes to a wide range of diameters.  My guess is that you'll
want to find someone pulling patch-clamp electrodes--those tend to be
about 1 micron--and then see what you can do by manipulating the pulling
parameters.  Or even easier, just take a too-small pipette and bump it
under microscopic control against a piece of glass--you can break the
tip to just about anything you need.

Good luck!

Martin Wessendorf

[hidden email] wrote:

> Hello everyone,
>
> I want to microinject human adipose stem cells with microbeads that
> range in size from 0.5-2.8 microns in diameter.  I am using a Narishige
> IM-9B microinjector.  This company does not sell pre-fabricated
> pipettes.  Can anyone recommend a company/brand of microinjection
> pipettes that are useful for microinjection of microbeads?  Thanks for
> any advice you can offer.
>
> Kris Ford
> Graduate Student
> Biomedical Engineering
> University of North Carolina, Chapel Hill
> Phone# [phone]

--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455
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