Posted by
Franco Del Principe on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Microinjection-pipettes-for-microbeads-tp2580788p2581005.html
Dear Kris and Martin
Martin's proposition is excellent.
Electrophysiologists have the appropriate borosilicate glass and
micropipette pullers. However, do NOT try to manipulate the parameters
yourself!!! Always ask an experienced user to pull them for you.
The puller is the electrophysiologist's sanctuary and the pipette's
shape and properties a science of its own. You mess with it and it's
likely you're going to be killed. More so if you break the heating
filament... ;-)
Just my 2 cents
Cheers
Franco
Martin Wessendorf wrote:
> Dear Kris--
>
> Rather than try to buy what you need, you might just go over to the
> Physiology Department in the medical school and ask who the
> electrophysiologists are. An electrophysiologist will have devices that
> can pull pipettes to a wide range of diameters. My guess is that you'll
> want to find someone pulling patch-clamp electrodes--those tend to be
> about 1 micron--and then see what you can do by manipulating the pulling
> parameters. Or even easier, just take a too-small pipette and bump it
> under microscopic control against a piece of glass--you can break the
> tip to just about anything you need.
>
> Good luck!
>
> Martin Wessendorf
>
>
[hidden email] wrote:
>> Hello everyone,
>>
>> I want to microinject human adipose stem cells with microbeads that
>> range in size from 0.5-2.8 microns in diameter. I am using a
>> Narishige IM-9B microinjector. This company does not sell
>> pre-fabricated pipettes. Can anyone recommend a company/brand of
>> microinjection pipettes that are useful for microinjection of
>> microbeads? Thanks for any advice you can offer.
>>
>> Kris Ford
>> Graduate Student
>> Biomedical Engineering
>> University of North Carolina, Chapel Hill
>> Phone# 919-962-3296
>
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