http://confocal-microscopy-list.275.s1.nabble.com/Microinjection-pipettes-for-microbeads-tp2580788p2581164.html
want to check with any C. Elegans labs at your institution. RNAi by
> Dear Kris--
> Rather than try to buy what you need, you might just go over to the
> Physiology Department in the medical school and ask who the
> electrophysiologists are. An electrophysiologist will have devices
> that can pull pipettes to a wide range of diameters. My guess is
> that you'll want to find someone pulling patch-clamp electrodes--
> those tend to be about 1 micron--and then see what you can do by
> manipulating the pulling parameters. Or even easier, just take a
> too-small pipette and bump it under microscopic control against a
> piece of glass--you can break the tip to just about anything you need.
> Good luck!
> Martin Wessendorf
>
[hidden email] wrote:
>> Hello everyone,
>>
>> I want to microinject human adipose stem cells with microbeads that
>> range in size from 0.5-2.8 microns in diameter. I am using a
>> Narishige IM-9B microinjector. This company does not sell pre-
>> fabricated pipettes. Can anyone recommend a company/brand of
>> microinjection pipettes that are useful for microinjection of
>> microbeads? Thanks for any advice you can offer.
>>
>> Kris Ford
>> Graduate Student
>> Biomedical Engineering
>> University of North Carolina, Chapel Hill
>> Phone# 919-962-3296