Posted by
Michal Gdula-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Correction-of-Z-axis-distortion-request-for-opinion-tp2580156p2593555.html
Thank you for your advice!
The price seems to be cheap, but I wander how it works with fluorescence. At
the moment probably I will stay with my vectashield so as to not chang to
many things in one go.
Regards,
Michal Gdula
Subject: Re: Correction of Z-axis distortion- request for opinion
From: Stanislav Vitha <
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Reply-To: Confocal Microscopy List
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Date: Sat, 4 Apr 2009 03:58:51 -0500
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I would also consider switching to a mounting medium with correct refractive
index (=same RI as immersion oil) 2,2-thiodiethanol comes to mind. See
Staudt, 2007. A 500 ml bottle can be had for ~$30.
1. Staudt, T., M.C. Lang, R. Medda, J. Engelhardt, and S.W. Hell, 2,2'-
thiodiethanol: a new water soluble mounting medium for high resolution optical
microscopy. Microsc Res Tech, 2007. 70(1): p. 1-9.
Stan Vitha
Microscopy and Imaging Center
Texas A&M University