Re: GFP and mCherry

Posted by Fred Mast on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Photobleaching-mechanism-question-tp2334316p2599503.html

It depends on the antibody. GFP and mCherry share similar residues in their N-terminus (the first 16 I believe). You can test this by conducting a western with your sample.

Cheers,

Fred

On 7-Apr-09, at 6:19 AM, Valeria Berno wrote:

Quick question:

Does someone knows if the antibody anti-GFP would cross-react also with the mCherry?

I assume the answer is no but i cannot find any specification. I could only found no cross-reactivity of DsRed antibodies with GFP......

Thanks in advance

Valeria

Fred D. Mast

Department of Cell Biology

Medical Sciences Building Room 5-14

University of Alberta

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Canada

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