Re: GFP and mCherry

Posted by vb-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Photobleaching-mechanism-question-tp2334316p2599603.html

Hi Valeria,

 

mCherry carries additional 7 aa peptides of GFP origin, i.e. identical to GFP, at both the N- and C-terminus of the protein. Thus, it could be a cross-reactivity.

 

mRFP1 is similar to mCherry, and does not carry GFP peptides suggesting you a simple solution to the problem.

 

Cheers,

 

Vitaly

NCI-Frederick,

301-846-6575

 

 

From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Fred Mast
Sent: Tuesday, April 07, 2009 10:11 AM
To: [hidden email]
Subject: Re: GFP and mCherry

 

 

It depends on the antibody. GFP and mCherry share similar residues in their N-terminus (the first 16 I believe). You can test this by conducting a western with your sample.

 

Cheers,

 

Fred

 

On 7-Apr-09, at 6:19 AM, Valeria Berno wrote:

Quick question:

Does someone knows if the antibody anti-GFP would cross-react also with the mCherry?

I assume the answer is no but i cannot find any specification. I could only found no cross-reactivity of DsRed antibodies with GFP......

Thanks in advance

Valeria

 

Fred D. Mast

Department of Cell Biology

Medical Sciences Building Room 5-14

University of Alberta

Edmonton, Alberta, T6G 2H7

Canada

 

Tel: [phone]

[hidden email]