Re: Rotate microscope to view vertical specimen

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Rotate-microscope-to-view-vertical-specimen-tp2636715p2648838.html

When you are operating horizontally you have to use springs to keep
tension on the gears at all times.  When you are vertical gravity does
this for you.

Craig


On Thu, Apr 16, 2009 at 11:31 AM, Richard E. Edelmann
<[hidden email]> wrote:

> We "rotated" a confocal a number of years ago.  This "Objective
> Inverter" seems like an easier way.
>
> The biggest issue is that the focus rack and gears are designed for a
> verticle motion.  There is A lot of whiplash when changing focus
> directions.  Our system has approximately 185 µm of slack before
> changing directions in focusing.  So you are pretty much lmited to
> focusing in one direction. For z-series you always have to reset the
> focus gearing since the normal stepers etc do not take into accoutn a
> the large whiplash.
>
> Oh, ours is an older PCM2000 on a Nikon Optiphot.
>
>
>
>
>
> On 15 Apr 2009 at 1:12, Stephen Cody wrote:
>
>> Dear Richard,
>>
>> A simple and fairly cost effective way to do it may be with the "Objective Inverter"
>> http://www.lsmtech.com/objectiveinverter.html
>>
>> The name implies inverting the objective. But I think it is capable of turning the objective to any angle.
>>
>> No commercial interest, and I have not used this system either, but it looks very cleaver.
>>
>> Cheers
>> Steve Cody
>>
>> 2009/4/15 Richard Superfine <[hidden email]<mailto:[hidden email]>>
>> We are interested in viewing a cell culture that has been turned vertically
>> so that the surface liquid can drain. We therefore need to rotate the
>> microscope so that its optical axis is horizontal. We want to be able to
>> rotate the microscope continuously from a vertical optical axis (normal
>> configuration) to a horizontal one. Does anyone know of a publication that
>> describes the mechanical design of a rotation assembly for accomplishing this?
>> Thanks,
>> Rich
>>
>> Richard Superfine
>> Bowman and Gordon Gray Professor
>> Department of Physics and Astronomy
>> Director, Center for Computer Integrated Systems for Microscopy and
>> Manipulation (CISMM) Phillips Hall CB3255 University of North Carolina
>> Chapel Hill, NC 27599-3255
>> 919.962.1185   CISMM.org
>>
>>
>>
>> --
>> Stephen H. Cody
>> Consulting Microscopist
>>
>
>
> Richard E. Edelmann, Ph.D.
> EXPO Editor, Microscopy and Microanalysis Supplement
> Electron Microscopy Facility Director
> 364 Pearson Hall
> Miami University, Oxford, OH 45056
> Ph: 513.529.5712        Fax: [phone]
> E-mail: [hidden email]
> http://www.emf.muohio.edu
>