Re: GFP tissue preparation for confocal microscopy

Posted by Alice Rodriguez Diaz on
URL: http://confocal-microscopy-list.275.s1.nabble.com/GFP-tissue-preparation-for-confocal-microscopy-tp2680696p2680807.html

Dear Young,

I don't think that paraformaldehyde should be the problem. Are you  
using methanol? if so, methanol does kill GFP. Depending on your sample,
consider switching to ethanol and related fixation protocols.

Best,
Alice

Dr Alice Rodriguez-Diaz
*Center for Biomedical Engineering *
*University of Texas Medical Branch *
*301 University Blvd. Rt. 1156, L17680*

*Research Support Building # 21*
*Galveston, Texas 77555-1156*
*Center Phone:  [phone]  **Fax:  [phone] *





Young Jik Kwon wrote:

> All,
>
> We are trying to take confocal micrographs of at tumor tissues that
> express GFP. What would be the sample preparation procedures? We tried
> cryosectioned samples but the cell morphology seems weird. We already
> know paraformaldehyde fixation kills GFP fluorescence. Any expert's
> advice?
>
> Best,
>
> Young
>
>