Re: GFP tissue preparation for confocal microscopy

Posted by RICHARD BURRY on
URL: http://confocal-microscopy-list.275.s1.nabble.com/GFP-tissue-preparation-for-confocal-microscopy-tp2680696p2682849.html

Young
 
Cryostat sections should appear like the live tissue.  If you are doing overnight infiltration with 20% sucrose in buffer and rapid freezing with isopentane then I have no idea what the problem is.  Most problems with morphology in Cryostat sections of paraformaldehyde fixed tissue comes from the freezing procedure.
 
Dick

----- Original Message -----
From: Young Jik Kwon <[hidden email]>
Date: Wednesday, April 22, 2009 10:44 pm
Subject: GFP tissue preparation for confocal microscopy
To: [hidden email]

> All,

> We are trying to take confocal micrographs of at tumor tissues that express GFP. What would be the sample preparation procedures? We tried cryosectioned samples but the cell morphology seems weird. We already know paraformaldehyde fixation kills GFP fluorescence. Any expert's advice?

> Best,

> Young

---

> Spam
> Not spam
> Forget previous vote



Richard W. Burry, Ph.D.
Department of Neuroscience, College of Medicine
Campus Microscopy and Imaging Facility, Director
The Ohio State University
Associate Editor, Journal of Histochemistry and Cytochemistry
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