Re: GFP tissue preparation for confocal microscopy

Posted by Stephen Bunnell on
URL: http://confocal-microscopy-list.275.s1.nabble.com/GFP-tissue-preparation-for-confocal-microscopy-tp2680696p2683883.html

PFA does not kill GFP fluorescence.

BAD PFA kills GFP fluorescence.

We routinely use 1% PFA with GFP, YFP, and CFP, and have never had a problem so long as the PFA is fresh and pH’ed to between 7.0 and 7.4.

    -Steve



On 4/22/09 10:34 PM, "Young Jik Kwon" <[hidden email]> wrote:

All,

We are trying to take confocal micrographs of at tumor tissues that express GFP. What would be the sample preparation procedures? We tried cryosectioned samples but the cell morphology seems weird. We already know paraformaldehyde fixation kills GFP fluorescence. Any expert's advice?

Best,

Young

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Stephen C. Bunnell, Ph.D.
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Tufts University Medical School
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