Re: GFP tissue preparation for confocal microscopy

Posted by anh2006 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/GFP-tissue-preparation-for-confocal-microscopy-tp2680696p2684246.html

Dear Caroline,

What do you mean by dehydration precisely? Do you dry your slides after sectioning?

Here is our ampoules info:

(1) http://www.alfa.com, Alfa Aesar, Item #43368

(2) http://www.emsdiasum.com, Electron Microscopy Sciences, Item # 15710

Thanks,

Andrea

At 12:05 PM -0400 4/23/09, Caroline Bass wrote:

I concur. I regularly perfuse rats and mice with formaldehyde, and I don't have any problem getting a signal (viral vector delivered EGFP in brain, spleen, liver, muscle, etc.). I have seen severe loss with any sort of dehydration or methanol fixation in fresh tissue.

Can someone recommend a good PFA? (Product number and company please!) The ampules sound interesting...

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