Posted by
allison van-de-meene (RRes-Roth) on
URL: http://confocal-microscopy-list.275.s1.nabble.com/digital-cameras-tp2846998p2861982.html
Hi Elaine,
We have a QImaging Retiga Exi monochrome camera with an RGB filter wheel that can slide in and out. It works really well for fluorescence and the colours for brightfield were the best we saw for our applications after testing a range of cameras.
Best wishes,
Allison
________________________________
From: Confocal Microscopy List on behalf of Cameron Nowell
Sent: Mon 5/11/2009 7:42 AM
To:
[hidden email]
Subject: Re: digital cameras
Hi Elaine,
I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders for the last 4 years. They work great, very sensitive for fluorescence and take good quality colour images. Basically you get two cameras in one. The software they use is simple and fairly intuitive to use. I don't think i have ever had a user say that it was difficult to use.
Cheers
Cam
Cameron J. Nowell
Microscopy Manager
Centre for Advanced Microscopy
Ludwig Institute for Cancer Research
PO Box 2008
Royal Melbourne Hospital
Victoria, 3050
AUSTRALIA
Office: +61 3 9341 3155
Mobile: +61422882700
Fax: +61 3 9341 3104
Facility Website <
http://www.ludwig.edu.au/branch/research/platform/microscopy.htm>
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Peter Pitrone
Sent: Monday, 11 May 2009 4:34 PM
To:
[hidden email]
Subject: Re: digital cameras
Hello Elaine,
I would recommend a SPOT RT Slider, or something similar. It is a Monochrome camera with a bayer filter slider that you can remove for fluorescence. Monochrome cameras are always better for fluorescence (bayer filters always eat a lot of light) with higher Quantum Efficiencies.
Pete
On May 8, 2009, at 8:51 PM, Elaine Kunze wrote:
One of our labs is planning to buy a color digital camera for bright-field and fluorescence pictures. They are considering the PAXcam2+ and Infinity X32. Does anyone have any experience with either of these two color cameras or any other camera suggestions?
Elaine Kunze
Cytometry Facility
Huck Institute of the Life Sciences
319 Life Sciences Building
Penn State University
University Park, PA 16802
http://www.huck.psu.edu/facilities/cytometry-up/814-863-2762
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