Re: digital cameras

Posted by Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/digital-cameras-tp2846998p2863819.html

Dear Elaine,
Along the same approach, CRI makes tunable filters that can be fitted  
to most microscopes to allow RGB brightfield images with any  
monochrome camera.  then you still have the ability for fluorescence,  
provided your camera has a QE curve and sufficient cooling for long  
exposures (or you have bright fluorescent labels).  Such filters can  
be readily controlled by most acquisition software and provide  
automatic white balance to set exposure times.  With most acquisition  
software, you can also set the fluorescent filters for sequential  
acquisition  of red, green and blue channels of a brightfield image.  
Although, white balance may not be automated.

Regards,
Glen

On May 10, 2009, at 11:42 PM, Cameron Nowell wrote:

> Hi Elaine,
>
> I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders  
> for the last 4 years. They work great, very sensitive for  
> fluorescence and take good quality colour images. Basically you get  
> two cameras in one. The software they use is simple and fairly  
> intuitive to use. I don’t think i have ever had a user say that it  
> was difficult to use.
>
>
> Cheers
>
> Cam
>
>
>
> Cameron J. Nowell
> Microscopy Manager
> Centre for Advanced Microscopy
> Ludwig Institute for Cancer Research
> PO Box 2008
> Royal Melbourne Hospital
> Victoria, 3050
> AUSTRALIA
>
> Office: [phone]
> Mobile: [phone]
> Fax: [phone]
>
> Facility Website
>
>
> From: Confocal Microscopy List [mailto:[hidden email]
> ] On Behalf Of Peter Pitrone
> Sent: Monday, 11 May 2009 4:34 PM
> To: [hidden email]
> Subject: Re: digital cameras
>
> Hello Elaine,
>
> I would recommend a SPOT RT Slider, or something similar. It is a  
> Monochrome camera with a bayer filter slider that you can remove for  
> fluorescence. Monochrome cameras are always better for fluorescence  
> (bayer filters  always eat a lot of light) with higher Quantum  
> Efficiencies.
>
> Pete
>
> On May 8, 2009, at 8:51 PM, Elaine Kunze wrote:
>
> One of our labs is planning to buy a color digital camera for bright-
> field and fluorescence pictures.   They are considering the PAXcam2+  
> and Infinity X32.  Does anyone have any experience with either of  
> these two color cameras or any other camera suggestions?
>
> Elaine Kunze
> Cytometry Facility
> Huck Institute of the Life Sciences
> 319 Life Sciences Building
> Penn State University
> University Park, PA 16802
> http://www.huck.psu.edu/facilities/cytometry-up/
> 814-863-2762
>
>
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Glen MacDonald
Core for Communication Research
Virginia Merrill Bloedel Hearing Research Center
Box 357923
University of Washington
Seattle, WA 98195-7923  USA
[phone]
[hidden email]

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