http://confocal-microscopy-list.275.s1.nabble.com/digital-cameras-tp2846998p2883657.html
We have a Spot-RT. Typically the LCD-RGB filters are not used for
it, and the camera is operated in monochrome mode for that. The Spot
- as in R,G, and B emission + DIC. However, the movement of the
wheels (Ludl)that can be changed without image registration issues. The
stack collection. All of this is currently run by Compix SimplePCI
> Steve, you are right, the SPOT RT uses a LCD.
>
> If one already has to capture 3 images, I am wondering if it would be a
> good idea to use a monochrome camera plus three R/G/B filters sitting in
> the fluorescence filter turret. The acquisition software can be set up in
> a way that it takes three images and changes the filters in between. Not
> the fastest solution, but offers high resolution and sensitivity w/o the
> need for an additional b/w camera. One just needs a filter turret and
> three filters...
>
> Michael
>
>
>> Just a minor correction for the archives more than anything else. If I'm
>> not
>> mistaken the SPOT RT slider does not use a Bayer Mosaic filter but an
>> LCD RGB color changing filter, so that three shot colour images may be
>> taken
>> at full chip resolution.
>>
>> Cheers
>> Steve Cody
>> 2009/5/12 David Barnes <
[hidden email]>
>>
>>> Thanks Brian, and on that note, the newer RGB Sliders from QImaging have
>>> three ports, a clear port for most fluro, a clear port w/ IR blocking,
>>> and
>>> the LCF port. In addition, the Q filter is easily rotatable with respect
>>> to
>>> the camera body, to eliminate polarization interference. The filter is
>>> controlled thru the Q camera, or can be used with other cameras via a
>>> CRI
>>> control box.
>>>
>>> dave
>>>
>>> On Mon, May 11, 2009 at 12:31 PM, Armstrong, Brian
>>> <
[hidden email]>wrote:
>>>
>>>> Elaine, we have both systems, that is: SPOT RT Slider, and a Retiga EXi
>>>> with a CRI Liquid Crystal Tunable Filter. I think both are viable
>>>> options. The SPOT system will most likely cost less and I like the SPOT
>>>> software which is easy to use and free. The Retiga/CRI system is a
>>>> better system in my humble opinion, and we operate this with Image Pro
>>>> Plus software.
>>>> Cheers,
>>>>
>>>>
>>>> Brian D Armstrong PhD
>>>> Light Microscopy Core Manager
>>>> Beckman Research Institute
>>>> City of Hope
>>>> Dept of Neuroscience
>>>> 1450 E Duarte Rd
>>>> Duarte, CA 91010
>>>> 626-256-4673 x62872
>>>>
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag>>>> ing/Pages/default.aspx
>>>> -----Original Message-----
>>>> From: Confocal Microscopy List
>>>> [mailto:
[hidden email]]
>>>> On Behalf Of Glen MacDonald
>>>> Sent: Monday, May 11, 2009 8:59 AM
>>>> To:
[hidden email]
>>>> Subject: Re: digital cameras
>>>>
>>>> Dear Elaine,
>>>> Along the same approach, CRI makes tunable filters that can be fitted
>>>> to most microscopes to allow RGB brightfield images with any
>>>> monochrome camera. then you still have the ability for fluorescence,
>>>> provided your camera has a QE curve and sufficient cooling for long
>>>> exposures (or you have bright fluorescent labels). Such filters can
>>>> be readily controlled by most acquisition software and provide
>>>> automatic white balance to set exposure times. With most acquisition
>>>> software, you can also set the fluorescent filters for sequential
>>>> acquisition of red, green and blue channels of a brightfield image.
>>>> Although, white balance may not be automated.
>>>>
>>>> Regards,
>>>> Glen
>>>>
>>>> On May 10, 2009, at 11:42 PM, Cameron Nowell wrote:
>>>>
>>>>> Hi Elaine,
>>>>>
>>>>> I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders
>>>>> for the last 4 years. They work great, very sensitive for
>>>>> fluorescence and take good quality colour images. Basically you get
>>>>> two cameras in one. The software they use is simple and fairly
>>>>> intuitive to use. I don't think i have ever had a user say that it
>>>>> was difficult to use.
>>>>>
>>>>>
>>>>> Cheers
>>>>>
>>>>> Cam
>>>>>
>>>>>
>>>>>
>>>>> Cameron J. Nowell
>>>>> Microscopy Manager
>>>>> Centre for Advanced Microscopy
>>>>> Ludwig Institute for Cancer Research
>>>>> PO Box 2008
>>>>> Royal Melbourne Hospital
>>>>> Victoria, 3050
>>>>> AUSTRALIA
>>>>>
>>>>> Office: +61 3 9341 3155
>>>>> Mobile: +61422882700
>>>>> Fax: +61 3 9341 3104
>>>>>
>>>>> Facility Website
>>>>>
>>>>>
>>>>> From: Confocal Microscopy List
>>>> [mailto:
[hidden email]
>>>>> ] On Behalf Of Peter Pitrone
>>>>> Sent: Monday, 11 May 2009 4:34 PM
>>>>> To:
[hidden email]
>>>>> Subject: Re: digital cameras
>>>>>
>>>>> Hello Elaine,
>>>>>
>>>>> I would recommend a SPOT RT Slider, or something similar. It is a
>>>>> Monochrome camera with a bayer filter slider that you can remove for
>>>>> fluorescence. Monochrome cameras are always better for fluorescence
>>>>> (bayer filters always eat a lot of light) with higher Quantum
>>>>> Efficiencies.
>>>>>
>>>>> Pete
>>>>>
>>>>> On May 8, 2009, at 8:51 PM, Elaine Kunze wrote:
>>>>>
>>>>> One of our labs is planning to buy a color digital camera for bright-
>>>>> field and fluorescence pictures. They are considering the PAXcam2+
>>>>> and Infinity X32. Does anyone have any experience with either of
>>>>> these two color cameras or any other camera suggestions?
>>>>>
>>>>> Elaine Kunze
>>>>> Cytometry Facility
>>>>> Huck Institute of the Life Sciences
>>>>> 319 Life Sciences Building
>>>>> Penn State University
>>>>> University Park, PA 16802
>>>>>
http://www.huck.psu.edu/facilities/cytometry-up/>>>>> 814-863-2762
>>>>>
>>>>>
>>>>> No virus found in this incoming message.
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>>>>>
>>>>> This communication is intended only for the named recipient and may
>>>>> contain information that is confidential, legally privileged or
>>>>> subject to copyright; the Ludwig Institute for Cancer Research Ltd
>>>>> does not waiver any rights if you have received this communication
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>>>>> The views expressed in this communication are those of the sender
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>>>>>
>>>>
>>>>
>>>> Glen MacDonald
>>>> Core for Communication Research
>>>> Virginia Merrill Bloedel Hearing Research Center
>>>> Box 357923
>>>> University of Washington
>>>> Seattle, WA 98195-7923 USA
>>>> (206) 616-4156
>>>>
[hidden email]
>>>>
>>>> ************************************************************************
>>>> ******
>>>> The box said "Requires WindowsXP or better", so I bought a Macintosh.
>>>> ************************************************************************
>>>> ******
>>>>
>>>>
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>>
>> --
>> Stephen H. Cody