http://confocal-microscopy-list.275.s1.nabble.com/digital-cameras-tp2846998p2883706.html
Ok, Glen mentioned that already. Forget about it then.
> the fluorescence filter turret. The acquisition software can be set up in
>> Just a minor correction for the archives more than anything else. If I'm
>> not
>> mistaken the SPOT RT slider does not use a Bayer Mosaic filter but an
>> LCD RGB color changing filter, so that three shot colour images may be
>> taken
>> at full chip resolution.
>>
>> Cheers
>> Steve Cody
>> 2009/5/12 David Barnes <
[hidden email]>
>>
>>> Thanks Brian, and on that note, the newer RGB Sliders from QImaging
>>> have
>>> three ports, a clear port for most fluro, a clear port w/ IR blocking,
>>> and
>>> the LCF port. In addition, the Q filter is easily rotatable with
>>> respect
>>> to
>>> the camera body, to eliminate polarization interference. The filter is
>>> controlled thru the Q camera, or can be used with other cameras via a
>>> CRI
>>> control box.
>>>
>>> dave
>>>
>>> On Mon, May 11, 2009 at 12:31 PM, Armstrong, Brian
>>> <
[hidden email]>wrote:
>>>
>>>> Elaine, we have both systems, that is: SPOT RT Slider, and a Retiga
>>>> EXi
>>>> with a CRI Liquid Crystal Tunable Filter. I think both are viable
>>>> options. The SPOT system will most likely cost less and I like the
>>>> SPOT
>>>> software which is easy to use and free. The Retiga/CRI system is a
>>>> better system in my humble opinion, and we operate this with Image Pro
>>>> Plus software.
>>>> Cheers,
>>>>
>>>>
>>>> Brian D Armstrong PhD
>>>> Light Microscopy Core Manager
>>>> Beckman Research Institute
>>>> City of Hope
>>>> Dept of Neuroscience
>>>> 1450 E Duarte Rd
>>>> Duarte, CA 91010
>>>> 626-256-4673 x62872
>>>>
http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imag>>>> ing/Pages/default.aspx
>>>> -----Original Message-----
>>>> From: Confocal Microscopy List
>>>> [mailto:
[hidden email]]
>>>> On Behalf Of Glen MacDonald
>>>> Sent: Monday, May 11, 2009 8:59 AM
>>>> To:
[hidden email]
>>>> Subject: Re: digital cameras
>>>>
>>>> Dear Elaine,
>>>> Along the same approach, CRI makes tunable filters that can be fitted
>>>> to most microscopes to allow RGB brightfield images with any
>>>> monochrome camera. then you still have the ability for fluorescence,
>>>> provided your camera has a QE curve and sufficient cooling for long
>>>> exposures (or you have bright fluorescent labels). Such filters can
>>>> be readily controlled by most acquisition software and provide
>>>> automatic white balance to set exposure times. With most acquisition
>>>> software, you can also set the fluorescent filters for sequential
>>>> acquisition of red, green and blue channels of a brightfield image.
>>>> Although, white balance may not be automated.
>>>>
>>>> Regards,
>>>> Glen
>>>>
>>>> On May 10, 2009, at 11:42 PM, Cameron Nowell wrote:
>>>>
>>>> > Hi Elaine,
>>>> >
>>>> > I concur with Peter. I have been using SPOT RT (and Pursuit) Sliders
>>>> > for the last 4 years. They work great, very sensitive for
>>>> > fluorescence and take good quality colour images. Basically you get
>>>> > two cameras in one. The software they use is simple and fairly
>>>> > intuitive to use. I don't think i have ever had a user say that it
>>>> > was difficult to use.
>>>> >
>>>> >
>>>> > Cheers
>>>> >
>>>> > Cam
>>>> >
>>>> >
>>>> >
>>>> > Cameron J. Nowell
>>>> > Microscopy Manager
>>>> > Centre for Advanced Microscopy
>>>> > Ludwig Institute for Cancer Research
>>>> > PO Box 2008
>>>> > Royal Melbourne Hospital
>>>> > Victoria, 3050
>>>> > AUSTRALIA
>>>> >
>>>> > Office: +61 3 9341 3155
>>>> > Mobile: +61422882700
>>>> > Fax: +61 3 9341 3104
>>>> >
>>>> > Facility Website
>>>> >
>>>> >
>>>> > From: Confocal Microscopy List
>>>> [mailto:
[hidden email]
>>>> > ] On Behalf Of Peter Pitrone
>>>> > Sent: Monday, 11 May 2009 4:34 PM
>>>> > To:
[hidden email]
>>>> > Subject: Re: digital cameras
>>>> >
>>>> > Hello Elaine,
>>>> >
>>>> > I would recommend a SPOT RT Slider, or something similar. It is a
>>>> > Monochrome camera with a bayer filter slider that you can remove for
>>>> > fluorescence. Monochrome cameras are always better for fluorescence
>>>> > (bayer filters always eat a lot of light) with higher Quantum
>>>> > Efficiencies.
>>>> >
>>>> > Pete
>>>> >
>>>> > On May 8, 2009, at 8:51 PM, Elaine Kunze wrote:
>>>> >
>>>> > One of our labs is planning to buy a color digital camera for
>>>> bright-
>>>> > field and fluorescence pictures. They are considering the PAXcam2+
>>>> > and Infinity X32. Does anyone have any experience with either of
>>>> > these two color cameras or any other camera suggestions?
>>>> >
>>>> > Elaine Kunze
>>>> > Cytometry Facility
>>>> > Huck Institute of the Life Sciences
>>>> > 319 Life Sciences Building
>>>> > Penn State University
>>>> > University Park, PA 16802
>>>> >
http://www.huck.psu.edu/facilities/cytometry-up/>>>> > 814-863-2762
>>>> >
>>>> >
>>>> > No virus found in this incoming message.
>>>> > Checked by AVG - www.avg.com
>>>> > Version: 8.5.325 / Virus Database: 270.12.23/2106 - Release Date:
>>>> > 05/10/09 07:02:00
>>>> >
>>>> >
>>>> > This communication is intended only for the named recipient and may
>>>> > contain information that is confidential, legally privileged or
>>>> > subject to copyright; the Ludwig Institute for Cancer Research Ltd
>>>> > does not waiver any rights if you have received this communication
>>>> > in error.
>>>> > The views expressed in this communication are those of the sender
>>>> > and do not necessarily reflect the views of the Ludwig Institute for
>>>> > Cancer Research Ltd.
>>>> >
>>>>
>>>>
>>>>
>>>> Glen MacDonald
>>>> Core for Communication Research
>>>> Virginia Merrill Bloedel Hearing Research Center
>>>> Box 357923
>>>> University of Washington
>>>> Seattle, WA 98195-7923 USA
>>>> (206) 616-4156
>>>>
[hidden email]
>>>>
>>>> ************************************************************************
>>>> ******
>>>> The box said "Requires WindowsXP or better", so I bought a Macintosh.
>>>> ************************************************************************
>>>> ******
>>>>
>>>>
>>>> ---------------------------------------------------------------------
>>>>
>>>> SECURITY/CONFIDENTIALITY WARNING:
>>>> This message and any attachments are intended solely for the
>>>> individual
>>>> or
>>>> entity to which they are addressed. This communication may contain
>>>> information that is privileged, confidential, or exempt from
>>>> disclosure
>>>> under applicable law (e.g., personal health information, research
>>>> data,
>>>> financial information). Because this e-mail has been sent without
>>>> encryption, individuals other than the intended recipient may be able
>>>> to
>>>> view the information, forward it to others or tamper with the
>>>> information
>>>> without the knowledge or consent of the sender. If you are not the
>>>> intended
>>>> recipient, or the employee or person responsible for delivering the
>>>> message
>>>> to the intended recipient, any dissemination, distribution or copying
>>>> of the
>>>> communication is strictly prohibited. If you received the
>>>> communication
>>>> in
>>>> error, please notify the sender immediately by replying to this
>>>> message
>>>> and
>>>> deleting the message and any accompanying files from your system. If,
>>>> due to
>>>> the security risks, you do not wish to receive further communications
>>>> via
>>>> e-mail, please reply to this message and inform the sender that you do
>>>> not
>>>> wish to receive further e-mail from the sender.
>>>> ---------------------------------------------------------------------
>>>>
>>>
>>>
>>
>>
>> --
>> Stephen H. Cody