Re: Recommendations for commercial multi-photon system purchase

Posted by Eric Scarfone on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Recommendations-for-commercial-multi-photon-system-purchase-tp2884074p2889852.html

Thanks for the precision Guy. You must be at least as old as I am!
2000 was about the time I installed the Biorad 1024 MP back in France. Did your Leica system actually worked back then (I mean with pico second laser, OS 9 and all?)    ;=)

Eric Scarfone, PhD, CNRS,
Center for Hearing and communication Research
Department of Clinical Neuroscience
Karolinska Institutet

Postal Address:
CFH, M1:02
Karolinska Hospital,
SE-171 76 Stockholm, Sweden

Work: [phone],
Cell: [phone]
Fax: [phone]

email: [hidden email]
http://www.ki.se/cfh/


----- Original Message -----
From: Guy Cox <[hidden email]>
Date: Thursday, May 14, 2009 3:15 am
Subject: Re: Recommendations for commercial multi-photon system purchase
To: [hidden email]

> Just to keep the record straight, our Leica MP system has non-

> descanneddetectors in both transmission and epi directions - this
> was delivered
> in 2000, ie 9 years ago. We were a fairly early customer for
> these. It
> was (IMHO) a better implementation than the Bio-Rad one (less
> sensitiveto room light). Zeiss back then did have non-descanned
> detection but
> only in transmitted direction - it worked quite well so long as your
> sample wasn't impossibly thick.
>
> Guy
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006

> ______________________________________________

> Phone [phone] Fax [phone]
> Mobile [phone] http://www.guycox.net
> ______________________________________________
>
>
> -----Original Message-----
> From: Confocal Microscopy List
> [mailto:[hidden email]]On Behalf Of Eric Scarfone
> Sent: Thursday, 14 May 2009 12:46 AM
> To: [hidden email]
> Subject: Re: Recommendations for commercial multi-photon system
> purchase
> Hi Lisa
> Lucky you to purchase such a machine!!
>
> The beauty with multiphoton excitation is that optical sectioning
> is
> achieved by the limited volume within which photon density is
> sufficient
> for 2 (or more) quasi simultaneous photon absorption events to
> take
> place. For a given laser setting, the thickness within which this

> occurs

> is only determined by the NA of your objective. ALL the
> fluorescence
> coming from your sample is emitted by this NA determined plane.
> There is
>
> no "out of focus" fluorescence. This is very different from Confocal.
>
> To make full use of this one would like to collect as much of the
> fluorescent photons emitted by the samples as possible. The most
> effective way to do that is to use non-descanned external
> detectors both
>
> in the reflected pathway and in the transmitted pathway.
>
> To my knowledge this was implemented first by Warren Zipfel in
> Watt Webb
>
> lab at Cornell and then commercialized by Bio-Rad on the last
> system
> they put on the market before diseappearing at the end of the
> previous
> century.... After a pretty long absorption/digestion process this
> was

> implemented rather recently by Zeiss.

> Others might have come up with similar solution but I do not know
> about
> it.
>
> I'd were you, for this level of price, I'd ask for an on site test
> with
> your own samples. Do not rely on "in factory" demos!!
>
> Cheers
> Eric
>
>
> Eric Scarfone, PhD, CNRS,
> Center for Hearing and communication Research
> Department of Clinical Neuroscience
> Karolinska Institutet
>
> Postal Address:
> CFH, M1:02
> Karolinska Hospital,
> SE-171 76 Stockholm, Sweden
>
> Work: [phone],
> Cell: [phone]
> Fax: [phone]
>
> email: [hidden email]
> http://www.ki.se/cfh/
>
>
> ----- Original Message -----
> From: "Cameron, Lisa" <[hidden email]>

> Date: Wednesday, May 13, 2009 4:14 pm

> Subject: Recommendations for commercial multi-photon system purchase
> To: [hidden email]
>
> > I have been investigating commercial multi-photon systems for
> > awhile in order to
> > purchase a system for my Institute's core microscopy facility.
> Our
> > interest is
> > to have the capability to do intravital imaging on an upright
> > stand, but also be
> > able to have facility users be able to put slides on and use the
> > visible scanner
> > and detectors. I realize this is a tall order for such
> versatility
> > in one
> > system, but since it is for a core (which needs to bring in
> > revenue), I'm
> > looking for the most flexible system. Does anyone have any
> > suggestions about the

> > most recent systems on the market? Or could you point out

> factors
> > you think are
> > the most important for making the decision on which company to
> go
> > with? Please
> > feel free to contact me off line.
> >
> > I have seen a demo of the Leica SP5 MP, Zeiss 710 NLO, Olympus
> MPE
> > and Prairie's
> > system.
> >
> > (BTW - my own experience is with widefield and confocal live-
> cell
> > imaging, so I
> > have not done 2-p myself, but have been learning about it for
> about
> > a year)
> >
> > Thanks!
> > - Lisa
> >
> > ---------------------------------------
> > Lisa Cameron, Ph.D.
> > Director of Confocal and Light Microscopy
> > Dana Farber Cancer Institute
> > 44 Binney St.; JF 215

> > Boston, MA 02115

> > Office phone: [phone]
> > Fax: [phone]
> > [hidden email]
> >
> >
> >
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