Posted by
Guy Cox on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Recommendations-for-commercial-multi-photon-system-purchase-tp2884074p2890119.html
Olympus are certainly not the only people to make such a lens. Leica have one (it's almost the size of a beer can!) but that's not much comfort if you are running a third-party system based on an Olympus stand. I guess there's a caveat emptor here - make sure if you are buying a third-party system on a "Big 4" stand that the microscope manufacturer will warrant to make all new stuff available to you. Or else buy elsewhere.
Guy
Optical Imaging Techniques in Cell Biology
by Guy Cox CRC Press / Taylor & Francis
http://www.guycox.com/optical.htm______________________________________________
Associate Professor Guy Cox, MA, DPhil(Oxon)
Electron Microscope Unit, Madsen Building F09,
University of Sydney, NSW 2006
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http://www.guycox.net-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Cameron, Lisa
Sent: Thursday, 14 May 2009 11:26 PM
To:
[hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase
Thanks to all for your comments on commercial 2-p systems.
As for the Olympus objective - the rep from the local distributor (which no longer is, since Olympus just went direct) said, Olympus has restricted sale of this "super objective" to people who have or purchase Olympus MPE systems only because they are (or do not want to) lose to the competition. Also something about having to manufacture these lens and not having them available for their own Olympus customers. Seems like if they just stepped up production, they could make money selling them to whomever...
Zeiss as put some effort into making a lens to compete with this - they have a 20x 1.0 WD=1.8 that they say is competing well. I have not compared these two objectives directly myself to be able to make any conclusions yet.
- Lisa
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Lisa Cameron, Ph.D.
Director of Confocal and Light Microscopy Dana Farber Cancer Institute
44 Binney St.; JF 215
Boston, MA 02115
Office phone: 617-582-8824
Fax: 617-582-8750
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Watkins, Simon C
Sent: Thursday, May 14, 2009 9:17 AM
To:
[hidden email]
Subject: Re: [CONFOCALMICROSCOPY] Recommendations for commercial multi-photon system purchase
the objective is considerably larger than the standard oly objectives. both in thread diameter and length so modifications of the stand using oly parts is needed too.
Simon C. Watkins Ph.D, FRCPath
Professor and Vice Chair, Cell Biology and Physiology Professor, Immunology Director, Center for Biologic Imaging BSTS 225, University of Pittsburgh 3500 Terrace St.
Pittsburgh PA 15261
Tel: 412-352-2277
Fax:412-648-2797
URL:
http://www.cbi.pitt.edu-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Adrian Smith
Sent: Thursday, May 14, 2009 7:58 AM
To:
[hidden email]
Subject: Re: Recommendations for commercial multi-photon system purchase
I don't believe there is any technical reason why the 25x Olympus will not work on the LaVision BioTec or Prairie systems.
However, my understanding is that Olympus will only sell that objective with a new Olympus MPE system... (which makes it a lot more than just "super expensive" :).
Extremely disappointing decision on Olympus' part.
I would absolutely love to try the objective on our Olympus microscope (supplied by LaVision BioTec) but I am unable to. If it performs as well as the marketing material suggests then I would be willing to pay a premium for it.
Regards,
Adrian Smith
Centenary Institute, Sydney, Australia
On 14/05/2009, at 9:23 PM, Watkins, Simon C wrote:
> If and when you try the olympus system make sure they bring you the
> super expensive dipping/coverslip water optic (25X1.12 NA) which they
> designed specifically for 2p. It is absolutely spectacular,
> and pretty much the only lens we use for all our mpe work nowadays.
> When you compare systems it may make you lean towards this particular
> manufacturer. Of course the LaVision and Prairie scan heads will fit
> on the Oly stand, not sure whether they will work with this lens but
> it would be great to find out (anyone out there tried this combo yet).
> S.
> Simon C. Watkins Ph.D, FRC Path
> Professor and Vice Chair Cell Biology and Physiology Professor
> Immunology Director Center for Biologic Imaging BSTS 225 University of
> Pittsburgh 3500 Terrace St Pittsburgh PA 15261
> 412-352-2277
> www.cbi.pitt.edu
>
> ________________________________________
> From: Confocal Microscopy List [
[hidden email]] On
> Behalf Of Adrian Smith [
[hidden email]]
> Sent: Wednesday, May 13, 2009 8:04 PM
> To:
[hidden email]
> Subject: Re: Recommendations for commercial multi-photon system
> purchase
>
> On 14/05/2009, at 1:57 AM, Alison North wrote:
>
>> If you were only interested in multiphoton imaging, the LaVision has
>> certain advantages, but if you need visible lasers too then I'm
>> afraid it won't suit your purposes.
>
> I'm not sure of the details but the LaVision BioTec did mention some
> confocal options to me in passing when we were discussing
> specifications recently. We were not interested so I didn't ask any
> details but it might be worth clarifying with them what they can
> currently offer.
>
> From recollection I think the Prairie was also MP-only, at least when
> we were looking (that was one of its selling points). Maybe that has
> changed or I'm misrembering?
>
> Regards,
>
> Adrian Smith
> Centenary Institute, Sydney, Australia
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