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Posted by Klara Balint on
URL: http://confocal-microscopy-list.275.s1.nabble.com/intensity-measurements-tp3005594p3007486.html

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Klara Balint
Postdoctoral Fellow
Dr. George Coukos` laboratory
University of Pennsylvania
431 Curie Blvd, BRB 2/3, Rm 1331
Philadelphia, PA 19130
Phone: [phone]
Fax: [phone]
Email: [hidden email]

----- "Guy Cox" <[hidden email]> wrote:

> In terms of quantitation, surely the best result is to forget RGB
> imaging altogether. From what I recall DAB gives a browny-black
> colour, so a greyscale image should do fine. But I do echo Julio's
> doubts about quantitation. If you are working in fluorescence, rather
> than picking one RGB channel the best thing is to get a filter (or set
> a spectral range) which matches your fluorochrome and excludes
> anything else, then again record an image of just that channel.
>
> Guy
>
>
>
> Optical Imaging Techniques in Cell Biology
> by Guy Cox CRC Press / Taylor & Francis
> http://www.guycox.com/optical.htm
> ______________________________________________
> Associate Professor Guy Cox, MA, DPhil(Oxon)
> Electron Microscope Unit, Madsen Building F09,
> University of Sydney, NSW 2006
> ______________________________________________
> Phone [phone] Fax [phone]
> Mobile [phone]