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> ----- "Guy Cox" <
[hidden email]> wrote:
>
>
>> In terms of quantitation, surely the best result is to forget RGB
>> imaging altogether. From what I recall DAB gives a browny-black
>> colour, so a greyscale image should do fine. But I do echo Julio's
>> doubts about quantitation. If you are working in fluorescence, rather
>> than picking one RGB channel the best thing is to get a filter (or set
>> a spectral range) which matches your fluorochrome and excludes
>> anything else, then again record an image of just that channel.
>>
>> Guy
>>
>>
>>
>> Optical Imaging Techniques in Cell Biology
>> by Guy Cox CRC Press / Taylor & Francis
>>
http://www.guycox.com/optical.htm>> ______________________________________________
>> Associate Professor Guy Cox, MA, DPhil(Oxon)
>> Electron Microscope Unit, Madsen Building F09,
>> University of Sydney, NSW 2006
>> ______________________________________________
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