plasmid for CFP-YFP tandem FRET

Posted by Jerry Sedgewick-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Perfect-Focus-tp1098084p3137196.html

Hello All,

A person using our lab is interested in the following:

We want to know if there's a commercially available plasmid to express a
CFP-YFP tandem FRET, with a linker protein that can be cleaved by
introducing a protease (that is non membrane-permeable). If one is not
commercially available, a lab that has produced this and would be
willing to share is also an option.

If anyone on the list has any experience with this/would be willing to
share, please let me know.  Thanks!

Jerry Sedgewick


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