Posted by
Miller, Jason on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Perfect-Focus-tp1098084p3144497.html
Just such a plasmid has been published by the Daugherty Group from UCSC, I believe (
http://www.ncbi.nlm.nih.gov/pubmed/15696158). It was published in Nature Biotechnology and they now have both bacterially optimized and mammalian optimized codon plasmids. The construct contains CyPet and Ypet (the evolutionarily optimized CFP and YFP derivatives that are supposed to FRET with each other with greater efficiency) with a Caspase 3 cleavage site in the middle.
-Jason
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-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Jerry Sedgewick
Sent: Monday, June 22, 2009 9:11 AM
To:
[hidden email]
Subject: plasmid for CFP-YFP tandem FRET
Hello All,
A person using our lab is interested in the following:
We want to know if there's a commercially available plasmid to express a
CFP-YFP tandem FRET, with a linker protein that can be cleaved by
introducing a protease (that is non membrane-permeable). If one is not
commercially available, a lab that has produced this and would be
willing to share is also an option.
If anyone on the list has any experience with this/would be willing to
share, please let me know. Thanks!
Jerry Sedgewick
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