Nuclear Dyes at 405nm?

Posted by Perry, Griffin M on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nuclear-Dyes-at-405nm-tp3144876.html

Dear Listserv,

 

I was wondering if anyone had better results with DAPI @ 405nm than I have.  It seems…off.  I know it is only getting excited @ 25%, but I know another group that gets good images at the same wavelength.  The staining has been uneven with DAPI, but with Topro-3 in the 638nm range, the staining has been beautiful with clearly defined nucleoli.  Does anybody use Hoechst 34580?    Are there any other alternatives to DAPI @ the 405nm range?

 

Thanks in advance,

 

-Griffin