Re: Nuclear Dyes at 405nm?

Posted by Rossi, Ann on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nuclear-Dyes-at-405nm-tp3144876p3144920.html

Griffin,

I have used Hoechst 34580 in live, freshly dissociated mouse skeletal muscle fibers (2.5ug/ml loaded 30 minutes at room temp). It was very bright with 405 laser excitation on the confocal.  Nuclear staining was beautiful.


-Ann Rossi
Postdoctoral Fellow
University of Chicago


-----Original Message-----
From: Confocal Microscopy List on behalf of Perry, Griffin M
Sent: Tue 6/23/2009 4:30 PM
To: [hidden email]
Subject: Nuclear Dyes at 405nm?
 
Dear Listserv,

 

I was wondering if anyone had better results with DAPI @ 405nm than I
have.  It seems...off.  I know it is only getting excited @ 25%, but I
know another group that gets good images at the same wavelength.  The
staining has been uneven with DAPI, but with Topro-3 in the 638nm range,
the staining has been beautiful with clearly defined nucleoli.  Does
anybody use Hoechst 34580?    Are there any other alternatives to DAPI @
the 405nm range?

 

Thanks in advance,

 

-Griffin