Re: Nuclear Dyes at 405nm?

Posted by Hong Zhou on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nuclear-Dyes-at-405nm-tp3144876p3145130.html

Please delist me, thanks a lot!
Hong

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From: "Rossi, Ann" <[hidden email]>
Sent: Tuesday, June 23, 2009 2:32 PM
To: <[hidden email]>
Subject: Re: Nuclear Dyes at 405nm?

> Griffin,
>
> I have used Hoechst 34580 in live, freshly dissociated mouse skeletal
> muscle fibers (2.5ug/ml loaded 30 minutes at room temp). It was very
> bright with 405 laser excitation on the confocal.  Nuclear staining was
> beautiful.
>
>
> -Ann Rossi
> Postdoctoral Fellow
> University of Chicago
>
>
> -----Original Message-----
> From: Confocal Microscopy List on behalf of Perry, Griffin M
> Sent: Tue 6/23/2009 4:30 PM
> To: [hidden email]
> Subject: Nuclear Dyes at 405nm?
>
> Dear Listserv,
>
>
>
> I was wondering if anyone had better results with DAPI @ 405nm than I
> have.  It seems...off.  I know it is only getting excited @ 25%, but I
> know another group that gets good images at the same wavelength.  The
> staining has been uneven with DAPI, but with Topro-3 in the 638nm range,
> the staining has been beautiful with clearly defined nucleoli.  Does
> anybody use Hoechst 34580?    Are there any other alternatives to DAPI @
> the 405nm range?
>
>
>
> Thanks in advance,
>
>
>
> -Griffin
>