Posted by
Gregg Sobocinski on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Nuclear-Dyes-at-405nm-tp3144876p3149064.html
LEAVING
Send an email to
[hidden email] (and NOT
[hidden email]) and put the following into the body of the message:
*signoff confocalmicroscopy* (note that it *isn't* "signoff confocal")
Alternatively, a subscriber can go to the website
http://lists.umn.edu/ and follow the directions on the web page.
Jerry
--
Jerry (Gerald) Sedgewick
Program Director, Biomedical Image Processing Lab (BIPL) Department of Neuroscience, University of Minnesota
312 Church St. SE, 1-205 Hasselmo Hall
Minneapolis, MN 55455
(612) 624-6607
[hidden email]
http://www.bipl.umn.edu-----Original Message-----
From: Confocal Microscopy List [mailto:
[hidden email]] On Behalf Of Hong Zhou
Sent: Tuesday, June 23, 2009 5:05 PM
To:
[hidden email]
Subject: Re: Nuclear Dyes at 405nm?
Please delist me, thanks a lot!
Hong
--------------------------------------------------
From: "Rossi, Ann" <
[hidden email]>
Sent: Tuesday, June 23, 2009 2:32 PM
To: <
[hidden email]>
Subject: Re: Nuclear Dyes at 405nm?
> Griffin,
>
> I have used Hoechst 34580 in live, freshly dissociated mouse skeletal
> muscle fibers (2.5ug/ml loaded 30 minutes at room temp). It was very
> bright with 405 laser excitation on the confocal. Nuclear staining was
> beautiful.
>
>
> -Ann Rossi
> Postdoctoral Fellow
> University of Chicago
>
>
> -----Original Message-----
> From: Confocal Microscopy List on behalf of Perry, Griffin M
> Sent: Tue 6/23/2009 4:30 PM
> To:
[hidden email]
> Subject: Nuclear Dyes at 405nm?
>
> Dear Listserv,
>
>
>
> I was wondering if anyone had better results with DAPI @ 405nm than I
> have. It seems...off. I know it is only getting excited @ 25%, but I
> know another group that gets good images at the same wavelength. The
> staining has been uneven with DAPI, but with Topro-3 in the 638nm range,
> the staining has been beautiful with clearly defined nucleoli. Does
> anybody use Hoechst 34580? Are there any other alternatives to DAPI @
> the 405nm range?
>
>
>
> Thanks in advance,
>
>
>
> -Griffin
>