Spacers for Inverted Microscop

Posted by Sam Albers on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spacers-for-Inverted-Microscop-tp3233585.html

Hello all,

My institution's confocal set up uses an inverted microscope. So when I view some biofilm samples I am collecting, I need to also invert my slide and place it in a NUNC coverslip chamber. Because I do not want to crush my biofilm sample, I need thin spacers to separate the slide from the coverslip bottom. Thin glass microscope slide have been suggested as a spacer. I was wondering if any one here had any other suggestions.

I am looking for a convenient inert object that can be be easily placed inside the coverslip chamber that will separate the slide from the coverslip bottom. Ideally, this would some type of material that I could vary the length to account for different thicknesses of biofilms.

Any suggestions would be greatly appreciated. I guess I am just wondering what other people do.

Thanks!

Sam