Posted by
John Oreopoulos on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spacers-for-Inverted-Microscop-tp3233585p3233631.html
You could try double sided tape layered on each other or wax. Grace
Biolabs also makes imaging chamber spacers (no commercial interest):
http://www.gracebio.com/Products/Imaging__Microscopy/
SecureSeal_Imaging_Spacers/
They make spacers with a variety of thicknesses, and I recall using
one version that was very thin.
John Oreopoulos
On 9-Jul-09, at 3:27 PM, Sam Albers wrote:
> Hello all,
>
> My institution's confocal set up uses an inverted microscope. So
> when I view
> some biofilm samples I am collecting, I need to also invert my
> slide and
> place it in a NUNC coverslip chamber. Because I do not want to
> crush my
> biofilm sample, I need thin spacers to separate the slide from the
> coverslip
> bottom. Thin glass microscope slide have been suggested as a
> spacer. I was
> wondering if any one here had any other suggestions.
>
> I am looking for a convenient inert object that can be be easily
> placed
> inside the coverslip chamber that will separate the slide from the
> coverslip
> bottom. Ideally, this would some type of material that I could vary
> the
> length to account for different thicknesses of biofilms.
>
> Any suggestions would be greatly appreciated. I guess I am just
> wondering
> what other people do.
>
> Thanks!
>
> Sam
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