Posted by
Glen MacDonald-2 on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spacers-for-Inverted-Microscop-tp3233585p3233750.html
You can get a diamond pencil and score coverslips into strips.
Coverslips may be purchased with thicknesses ranging from about 90
microns to 250 microns. Each thickness type has a 30 um range. If
you would need greater accuracy, a micrometer would be required to
measure the thickness.
Regards,
Glen
On Jul 9, 2009, at 12:27 PM, Sam Albers wrote:
> Hello all,
>
> My institution's confocal set up uses an inverted microscope. So
> when I view
> some biofilm samples I am collecting, I need to also invert my slide
> and
> place it in a NUNC coverslip chamber. Because I do not want to crush
> my
> biofilm sample, I need thin spacers to separate the slide from the
> coverslip
> bottom. Thin glass microscope slide have been suggested as a spacer.
> I was
> wondering if any one here had any other suggestions.
>
> I am looking for a convenient inert object that can be be easily
> placed
> inside the coverslip chamber that will separate the slide from the
> coverslip
> bottom. Ideally, this would some type of material that I could vary
> the
> length to account for different thicknesses of biofilms.
>
> Any suggestions would be greatly appreciated. I guess I am just
> wondering
> what other people do.
>
> Thanks!
>
> Sam
> --
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