Posted by
Deanne Veronica Catmull on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spacers-for-Inverted-Microscop-tp3233585p3235139.html
Why can't you grow your biofilm in a flow cell or a slide chamber? These
can be easily imaged via the Confocal without all the fuss of designing
and using these "spacers"? This is just a thought.
Kind regards,
Deanne.
-----Original Message-----
From: Confocal Microscopy List [mailto:
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On Behalf Of Sam Albers
Sent: Friday, 10 July 2009 5:27 AM
To:
[hidden email]
Subject: Spacers for Inverted Microscop
Hello all,
My institution's confocal set up uses an inverted microscope. So when I
view
some biofilm samples I am collecting, I need to also invert my slide and
place it in a NUNC coverslip chamber. Because I do not want to crush my
biofilm sample, I need thin spacers to separate the slide from the
coverslip
bottom. Thin glass microscope slide have been suggested as a spacer. I
was
wondering if any one here had any other suggestions.
I am looking for a convenient inert object that can be be easily placed
inside the coverslip chamber that will separate the slide from the
coverslip
bottom. Ideally, this would some type of material that I could vary the
length to account for different thicknesses of biofilms.
Any suggestions would be greatly appreciated. I guess I am just
wondering
what other people do.
Thanks!
Sam
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