Re: Spacers for Inverted Microscop

Posted by Raman Rajagopal on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spacers-for-Inverted-Microscop-tp3233585p3238537.html

Why don't you try glass wool. They are inert and come in different sizes.

They have been routinely used in observing nematodes and other fragile invertebrates.

Alternatively, you could use thin wall Petri-plates used for live cell imaging, to prepare your samples. This can viewed directly ie without inverting.

Best wishes

RAJAGOPAL

University of Delhi

 

On Fri, Jul 10, 2009 at 12:57 AM, Sam Albers <[hidden email]> wrote:

Hello all,

My institution's confocal set up uses an inverted microscope. So when I view
some biofilm samples I am collecting, I need to also invert my slide and
place it in a NUNC coverslip chamber. Because I do not want to crush my
biofilm sample, I need thin spacers to separate the slide from the coverslip
bottom. Thin glass microscope slide have been suggested as a spacer. I was
wondering if any one here had any other suggestions.

I am looking for a convenient inert object that can be be easily placed
inside the coverslip chamber that will separate the slide from the coverslip
bottom. Ideally, this would some type of material that I could vary the
length to account for different thicknesses of biofilms.

Any suggestions would be greatly appreciated. I guess I am just wondering
what other people do.

Thanks!

Sam
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