Re: positive control for apoptosis

Posted by Gregg Sobocinski on
URL: http://confocal-microscopy-list.275.s1.nabble.com/positive-control-for-apoptosis-tp3240335p3240415.html

Do you have access to lactating rats? I've used post-lactation rat mammary glands. I believe the time period was 2-10 days post-lactation, but am not positive. Send me a personal message if you want me to look up the exact range for harvesting.

Regards,
~Gregg
 
Gregg Sobocinski
Microscope Imaging Specialist
University of Michigan, MCDB Dept.
Ann Arbor, Michigan
USA

-----Original Message-----
From: Confocal Microscopy List [mailto:[hidden email]] On Behalf Of Martin Wessendorf
Sent: Friday, July 10, 2009 1:58 PM
To: [hidden email]
Subject: positive control for apoptosis

Dear all--

Can anyone suggest a good tissue to use as a positive control for
apoptosis?  We've considered liver and testes but as nearly as I can
find from the literature, the proportion of apoptotic cells in those
organs in naive rats is pretty small.  Any other suggestions?

Thanks!

Martin Wessendorf
--
Martin Wessendorf, Ph.D.                   office: [phone]
Assoc Prof, Dept Neuroscience                 lab: [phone]
University of Minnesota             Preferred FAX: [phone]
6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
Minneapolis, MN  55455                    e-mail: [hidden email]