Re: positive control for apoptosis

Posted by Caroline Miller on
URL: http://confocal-microscopy-list.275.s1.nabble.com/positive-control-for-apoptosis-tp3240335p3240717.html

If you are doing tunel you can make your own by treating the sections  
with DNase prior to staining......otherwise I might try to find a  
decent tumour sample, with a little necrosis.

Happy Friday!
Caroline


Caroline Miller M.Sc DIC AIBMS
Co-manager
Gladstone Microscopy and Histology Core
J David Gladstone institute
1650 Owens Street
San Francisco
CA
94158

www.gladstone.ucsf.edu

On Jul 10, 2009, at 11:20, "Sobocinski, Gregg" <[hidden email]>  
wrote:

> Do you have access to lactating rats? I've used post-lactation rat  
> mammary glands. I believe the time period was 2-10 days post-
> lactation, but am not positive. Send me a personal message if you  
> want me to look up the exact range for harvesting.
>
> Regards,
> ~Gregg
>
> Gregg Sobocinski
> Microscope Imaging Specialist
> University of Michigan, MCDB Dept.
> Ann Arbor, Michigan
> USA
>
> -----Original Message-----
> From: Confocal Microscopy List  
> [mailto:[hidden email]] On Behalf Of Martin  
> Wessendorf
> Sent: Friday, July 10, 2009 1:58 PM
> To: [hidden email]
> Subject: positive control for apoptosis
>
> Dear all--
>
> Can anyone suggest a good tissue to use as a positive control for
> apoptosis?  We've considered liver and testes but as nearly as I can
> find from the literature, the proportion of apoptotic cells in those
> organs in naive rats is pretty small.  Any other suggestions?
>
> Thanks!
>
> Martin Wessendorf
> --
> Martin Wessendorf, Ph.D.                   office: [phone]
> Assoc Prof, Dept Neuroscience                 lab: [phone]
> University of Minnesota             Preferred FAX: [phone]
> 6-145 Jackson Hall, 321 Church St. SE    Dept Fax: [phone]
> Minneapolis, MN  55455                    e-mail: [hidden email]