Re: VisEn Prosense 750 on multiphoton?

Posted by Craig Brideau on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spacers-for-Inverted-Microscop-tp3233585p3265955.html

For multiphoton (in this case 2 photon) won't you need a very long
wavelength?  Probably >1200nm?  Ti:Saphs don't go beyond 1100nm so you
won't be able to use multiphoton to stimulate it unless you have a
Cr:Forsterite laser lying around or an OPA/OPO system strapped to your
Ti:Saph.  If you want to use single photon you can just tune your
Ti:Saph to 750 and you should be good to go.

Craig


On Wed, Jul 15, 2009 at 1:30 PM, Gastinger, Matthew (NIH/NIAID)
[C]<[hidden email]> wrote:

> We have someone who wants to use ProSense 750 (a protease activatable
> fluorescent in vivo imaging agent) from VisEN.  He wants to image in vivo
> using confocal microscopy.  The alternative is ProSense 680, but 750 is
> likely to produce less autofluorescence.  ProSense 680 will likely work with
> 633 laser or whitelight laser at 670nm.
>
>
>
> Has anyone imaged VisEN ProSense 750 using multiphoton confocal microscopy?
> If so, what are equipment specs generating images.
>
>
>
> Thanks.