Re: VisEn Prosense 750 on multiphoton?
Posted by
Andreas Bruckbauer on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spacers-for-Inverted-Microscop-tp3233585p3266037.html
>If you want to use single photon you can just tune your Ti:Saph to 750 and you should be good to go
You would need a suitable dichroic for direct excitation at 750 nm, the multiphoton dichroics usually reflect everything above 680 or so, your fluorescence signal would not reach the detector. Furthermore the normal photomultiplier detectors are almost blind in the far red, laser power would need to be reduced quite a lot... Has anyone tried direct 1P excitation of these dyes?
Andreas
-----Original Message-----
From: Craig Brideau <
[hidden email]>
To:
[hidden email]
Sent: Wed, 15 Jul 2009 22:37
Subject: Re: VisEn Prosense 750 on multiphoton?
For multiphoton (in this case 2 photon) won't you need a very long
wavelength? Probably >1200nm? Ti:Saphs don't go beyond 1100nm so you
won't be able to use multiphoton to stimulate it unless you have a
Cr:Forsterite laser lying around or an OPA/OPO system strapped to your
Ti:Saph. If you want to use single photon you can just tune your
Ti:Saph to 750 and you should be good to go.
Craig
On Wed, Jul 15, 2009 at 1:30 PM, Gastinger, Matthew (NIH/NIAID)
[C]<[hidden email]> wrote:
> We have someone who wants to use ProSense 750 (a protease activatable
> fluorescent in vivo imaging agent) from VisEN. He wants to image in vivo
> using confocal microscopy. The alternative is ProSense 680, but 750 is
> likely to produce less autofluorescence. ProSense 680 will likely work with
> 633 laser or whitelight laser at 670nm.
>
>
>
> Has anyone imaged VisEN ProSense 750 using multiphoton confocal microscopy?
> If so, what are equipment specs generating images.
>
>
>
> Thanks.