Re: VisEn Prosense 750 on multiphoton?
Posted by
Rainer Kohler-2 on
Jul 16, 2009; 12:48pm
URL: http://confocal-microscopy-list.275.s1.nabble.com/Spacers-for-Inverted-Microscop-tp3233585p3271403.html
Re: VisEn Prosense 750 on
multiphoton?
HI,
we use VIsen probes such as Prosense and Angiosense 680 and 750 a
lot in laser scanning microscopy with the Olympus IV100.
The IV100 has a laser for 748 nm excitation and appropriate
filters.
I have not tried multi photon imaging of these probes yet.
Rainer
At 6:03 PM -0400 7/15/09, Andreas Bruckbauer wrote:
>If you want
to use single photon you can just tune your Ti:Saph to 750 and you
should be good to go
You would need a suitable dichroic for direct
excitation at 750 nm, the multiphoton dichroics usually reflect
everything above 680 or so, your fluorescence signal would not reach
the detector. Furthermore the normal photomultiplier detectors are
almost blind in the far red, laser power would need to be reduced
quite a lot... Has anyone tried direct 1P excitation of these
dyes?
Andreas
-----Original Message-----
From: Craig Brideau <[hidden email]>
To: [hidden email]
Sent: Wed, 15 Jul 2009 22:37
Subject: Re: VisEn Prosense 750 on multiphoton?
For multiphoton (in this case 2
photon) won't you need a very long
wavelength? Probably >1200nm? Ti:Saphs don't go beyond
1100nm so you
won't be able to use multiphoton to stimulate it unless you have a
Cr:Forsterite laser lying around or an OPA/OPO system strapped to
your
Ti:Saph. If you want to use single photon you can just tune
your
Ti:Saph to 750 and you should be good to go.
Craig
On Wed, Jul 15, 2009 at 1:30 PM, Gastinger, Matthew (NIH/NIAID)
[C]<[hidden email]> wrote:
> We have someone who wants to use ProSense 750 (a protease
activatable
> fluorescent in vivo imaging agent) from VisEN. He wants to
image in vivo
> using confocal microscopy. The alternative is ProSense 680,
but 750 is
> likely to produce less autofluorescence. ProSense 680 will
likely work with
> 633 laser or whitelight laser at 670nm.
>
>
>
> Has anyone imaged VisEN ProSense 750 using multiphoton confocal
microscopy?
> If so, what are equipment specs generating images.
>
>
>
> Thanks.
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--
Rainer
Kohler, Ph.D
Assistant in Research
Cell Phone: 978 578 5057
E.mail: [hidden email]
Center for Systems Biology
Massachusetts General Hospital
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