Re: resolution target
Posted by
Rosemary.White on
URL: http://confocal-microscopy-list.275.s1.nabble.com/resolution-target-tp3298871p3300960.html
Re: resolution target
Another way to test optics is to coat a clean, but not ultraclean, glass slide with a reasonable amount of gold (i.e. thicker than you’d normally use for SEM) in a standard sputter coater, then wipe the coated surface very gently. This will produce tiny pinholes, just select the size you want to use. To preserve the surface, glue a long coverslip on top with your preferred mountant, that way you have the correct amount of glass between objective and object. It’s a good way of testing chromatic aberration, for example.
cheers,
Rosemary
Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601
Australia
ph 61 2 6246 5475
fx 61 2 6246 5334
On 22/07/09 1:49 AM, "Julio Vazquez" <jvazquez@...> wrote:
Hi Thomas,
Mr Klaus Kemp in the UK used to make diatom slides, including one with 8 species of diatoms covering a broad range of resolutions. The slides were about 20 Euros or so. I am not sure whether he is still in business though. Below is his web site, and the list of diatoms on his 8-form test plate.
Julio.
http://www.diatoms.co.uk/
- Amphipleura pellucida 270 nm
- Frustulia rhomboides 300 nm
- Pleurosigma angulatum 525 nm
- Surirella gemma 500 nm
- Nitzschia sigma 440 nm
- Stauroneis phoenicentron 720 nm
- Navicula lyra 1250 nm
- Gyrosigma balticum 670 nm
There also used to be an article by Dave Walker: Counting the dots: Giving Microscopes a workout using diatom test slides at
www.microscopy-uk.org.uk/mag/artoct99/dwdiatom.html
the article has been inaccessible for a while though
--
Julio Vazquez
Fred Hutchinson Cancer Research Center
Seattle, WA 98109-1024
http://www.fhcrc.org
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On Jul 21, 2009, at 5:11 AM, Thomas Aabo wrote:
Hi
Does anyone know a good (cheap) place to get a positive high resolution target for a bright field microscope (50X, N.A. 0.55). Inside the EU would be best.
Thanks
Thomas
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Thomas Aabo
Phone: +45 35333636
M.Sc., Ph.D student, taa@...
University of Copenhagen
Department of Food Science
Rolighedsvej 30
1958 Frederiksberg C
Denmark
www.ifv.life.ku.dk
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