Re: time-domain FLIM-FRET with fixed samples

Posted by Alessandro Esposito on
URL: http://confocal-microscopy-list.275.s1.nabble.com/Re-3D-Eroded-Object-Masks-commercial-response-tp3272647p3302126.html

Dear Guy,
   we fully agree, I may have explained myself not correctly. I was just
mentioning that time-gating with a few time windows is slightly less photon
efficient, but can provide faster acquisition times. It provide less information
content per counted photons, but can count a higher number of photons.

It is a couple of years that B&H claims thay can detect lifetimes that fast, but
I guess this can happen only with their multi-detector system and I personally
never met anybody that got a TCSPC (good) image in a few seconds.

Cheers,

Alessandro